To investigate the restriction enzyme that discriminates between hba (normal) and hbs (disease) genes and perform a simulated test on a patient
Genetically inherited diseases in humans can be caused by a single nucleotide mutation resulting in a change
Such a substitution can affect the structure and function of a critical protein, rendering it partially or completely nonfunctional
A classic example is hemoglobin in sickle cell anemia
Agarose gel-based experiment designed for DNA staining and visualization with InstaStain methylene blue
Demonstrates the use of a restriction enzyme that discriminates between the normal and sickle cell hemoglobin genes
This experiment does not contain human DNA
Requires electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system
Includes: Instructions, Ready-to-load Quickstrip DNA samples, Ultraspec agarose powder, practice gel loading solution, electrophoresis buffer, Instastain blue and Flashblue
